Real-time RT-PCR high-resolution melting curve analysis to detect and differentiate Brazilian variants of grapevine viruses
Detecting and identifying viral infections in perennial plants, such as grapevines, can be challenging.Therefore, the aim of this study was to perform a real-time RT-PCR (RT-qPCR) high-resolution melting (HRM) curve analysis to detect and differentiate Brazilian variants of grapevine leafroll-associated strikketøy oppbevaring virus 3 (GLRaV-3) and